From b19970fb4855b85a35d5b414a303da6f66831c35 Mon Sep 17 00:00:00 2001 From: Xi Chen Date: Thu, 7 Mar 2024 00:03:16 +0800 Subject: [PATCH] clean up split-seq --- methods_html/SPLiT-seq.html | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/methods_html/SPLiT-seq.html b/methods_html/SPLiT-seq.html index f1a3fcf..c60ae8e 100644 --- a/methods_html/SPLiT-seq.html +++ b/methods_html/SPLiT-seq.html @@ -9,11 +9,11 @@

SPLiT-seq / microSPLiT

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The SPLiT-seq uses the combinatorial indexing to identify single cells without single cell isolation. Multi-level indexing can be performed by ligations. The workflow described here is based on the publication of Science 360, 176-182 (2018). To be consistent with the publication, all the oligo/primer names are the same as the Science 2018 publication. All oligos can be found in the Supplementary Table S12 from the publication. NOTE: The published version in this page is different from the preprint version in the bioRxiv. I have archived the previous Github Page about SPLiT-seq, which was based on the preprint version. If you want to have a look, you can check by clicking here. +

The SPLiT-seq uses the combinatorial indexing to identify single cells without single cell isolation. Multi-level indexing can be performed by ligations. The workflow described here is based on the publication of Science 360, 176-182 (2018). To be consistent with the publication, all the oligo/primer names are the same as the Science 2018 publication. All oligos can be found in the Supplementary Table S12 from the publication. NOTE: The published version in this page is different from the preprint version in the bioRxiv. I have archived the previous Github Page about SPLiT-seq, which was based on the preprint version. If you want to have a look, you can check by clicking here.

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Be careful that there are different versions of the protocol, and they use slightly different oligo sequences. The basic idea of the method does not change. Check this thread for more information.

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Be careful that there are different versions of the protocol, and they use slightly different oligo sequences. The basic idea of the method does not change. Check this thread for more information.

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microSPLiT was based on SPLiT-seq, and it was developed from the same lab that developed SPLiT-seq. microSPLiT was further optimised to work on sequencing mRNA from bacteria. The oligo sequences used in microSPLiT is almost the same as those in SPLiT-seq, except that the Round2 linker region is shorter. Therefore, the final library of microSPLiT is extremely similar to the original SPLiT-seq. The procedures described here is based on the Science paper and the oligo sequences are taken from their Supplementary Table S3.

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microSPLiT was based on SPLiT-seq, and it was developed from the same lab that developed SPLiT-seq. microSPLiT was further optimised to work on sequencing mRNA from bacteria. The oligo sequences used in microSPLiT is almost the same as those in SPLiT-seq, except that the Round2 linker region is shorter. Therefore, the final library of microSPLiT is extremely similar to the original SPLiT-seq. The procedures described here is based on the Science paper and the oligo sequences are taken from their Supplementary Table S3.