diff --git a/topics/imaging/tutorials/detection-of-mitoflashes/data-library.yaml b/topics/imaging/tutorials/detection-of-mitoflashes/data-library.yaml
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+++ b/topics/imaging/tutorials/detection-of-mitoflashes/data-library.yaml
@@ -0,0 +1,19 @@
+---
+destination:
+  type: library
+  name: GTN - Material
+  description: Galaxy Training Network Material
+  synopsis: Galaxy Training Network Material. See https://training.galaxyproject.org
+items:
+- name: Imaging
+  description: Image analysis using Galaxy
+  items:
+  - name: Tracking of mitochondria and capturing mitoflashes
+    items:
+    - name: 'DOI: 10.5281/zenodo.14071552'
+      description: latest
+      items:
+      - url: https://zenodo.org/record/14071552/files/mitoflashes_8bit.tiff
+        src: url
+        ext: 
+        info: https://doi.org/10.5281/zenodo.14071552