From 39e705b0f0b798be1214f1025bf06297525711bf Mon Sep 17 00:00:00 2001 From: Jeffrey Grover Date: Tue, 6 Aug 2019 15:46:01 -0700 Subject: [PATCH] Removed unnecessary rule dependencies for better parallelization in anticipation of release --- Snakefile | 15 +++++++-------- dag.png | Bin 66199 -> 77797 bytes 2 files changed, 7 insertions(+), 8 deletions(-) diff --git a/Snakefile b/Snakefile index dc77f41..2ff4e79 100644 --- a/Snakefile +++ b/Snakefile @@ -12,7 +12,12 @@ REFERENCE_GENOME = config['reference_genome'] rule all: input: expand('6_mosdepth/{sample}.sorted.markdupes.coverage.txt', - sample=SAMPLES) + sample=SAMPLES), + expand('5_methyldackel_extract/{sample}.sorted.markdupes_{context}.{ext}', + sample=SAMPLES, context=['CpG', 'CHG', 'CHH'], + ext=['bedGraph', 'methylKit']), + expand('2_trim_galore/{sample}_R{mate}_val_{mate}_fastqc.{ext}', + sample=SAMPLES, mate=[1, 2], ext=['html', 'zip']) # Index the reference genome @@ -110,10 +115,6 @@ rule fastqc_trimmmed: rule bwameth_align: input: {rules.bwameth_index.output}, - '2_trim_galore/{sample}_R1_val_1_fastqc.html', - '2_trim_galore/{sample}_R1_val_1_fastqc.zip', - '2_trim_galore/{sample}_R2_val_2_fastqc.html', - '2_trim_galore/{sample}_R2_val_2_fastqc.zip', R1 = '2_trim_galore/{sample}_R1_val_1.fq.gz', R2 = '2_trim_galore/{sample}_R2_val_2.fq.gz' output: @@ -282,9 +283,8 @@ rule methyldackel_extract: # Get the depth for each sample -rule get_depth: +rule mosdepth: input: - {rules.methyldackel_extract.output}, bam = '3_aligned_sorted_markdupes/{sample}.sorted.markdupes.bam' output: '6_mosdepth/{sample}.sorted.markdupes.mosdepth.global.dist.txt', @@ -311,7 +311,6 @@ rule get_depth: # Calculate the coverage from the mosdepth output rule calc_coverage: input: - {rules.get_depth.output}, bed = '6_mosdepth/{sample}.sorted.markdupes.per-base.bed.gz' output: '6_mosdepth/{sample}.sorted.markdupes.coverage.txt' diff --git a/dag.png b/dag.png index 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