diff --git a/README.md b/README.md index 047824d..b7c47c9 100644 --- a/README.md +++ b/README.md @@ -1,6 +1,6 @@ ## yeastDrop-Seq Computational Pipeline -This repository contains scripts for data pre-processing and downstream analysis in the [yeastDrop-Seq paper]. +This repository contains scripts for data pre-processing and downstream analysis in the [yeastDrop-Seq paper](#). ### Required softwares @@ -11,3 +11,19 @@ This repository contains scripts for data pre-processing and downstream analysis [STAR](https://github.com/alexdobin/STAR) [Trimmomatic](http://www.usadellab.org/cms/?page=trimmomatic) [subread](http://subread.sourceforge.net/) + +#### Downstream analysis +[SC3](https://bioconductor.org/packages/release/bioc/html/SC3.html) +[SingleCellExperiment](https://bioconductor.org/packages/release/bioc/html/SingleCellExperiment.html) +[Seurat](https://cran.r-project.org/web/packages/Seurat/index.html) +[ggplot2](https://cran.r-project.org/web/packages/ggplot2/index.html) +[scales](https://cran.r-project.org/web/packages/scales/index.html) +[ggpubr](https://cran.r-project.org/web/packages/ggpubr/index.html) +[cowplot](https://cran.r-project.org/web/packages/cowplot/index.html) +[RColorBrewer](https://cran.r-project.org/web/packages/RColorBrewer/) +[networkD3](https://cran.r-project.org/web/packages/networkD3/index.html) +[dplyr](https://cran.r-project.org/web/packages/dplyr/index.html) +[patchwork](https://cran.r-project.org/web/packages/patchwork/) +[reshape2](https://cran.r-project.org/web/packages/reshape2/index.html) +[clusterProfiler](https://bioconductor.org/packages/release/bioc/html/clusterProfiler.html) +[org.Sc.sgd.db](https://bioconductor.org/packages/release/data/annotation/html/org.Sc.sgd.db.html) \ No newline at end of file diff --git a/in_house_scripts/wrapper.R b/in_house_scripts/wrapper.R index ebfc946..0eaae10 100644 --- a/in_house_scripts/wrapper.R +++ b/in_house_scripts/wrapper.R @@ -11,7 +11,6 @@ require(RColorBrewer) require(networkD3) require(dplyr) require(patchwork) -require(WebGestaltR) library(reshape2) library(clusterProfiler) library(org.Sc.sgd.db)