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In main.nf, both pre- and post-fastqc is run on the raw, untrimmed reads. spoligo_post is the raw reads, not the trimmed reads (fastp_reads1) from fastp. Furthermore, fastp_reads1 is not used anywhere else in the file. spoligo_post should be removed from the channels.into in Line 184.
Line 247-248:
input:
tuple file(trim1), file(trim2) from spoligo_post
The text was updated successfully, but these errors were encountered:
In main.nf, both pre- and post-fastqc is run on the raw, untrimmed reads. spoligo_post is the raw reads, not the trimmed reads (fastp_reads1) from fastp. Furthermore, fastp_reads1 is not used anywhere else in the file. spoligo_post should be removed from the channels.into in Line 184.
Line 247-248:
input:
tuple file(trim1), file(trim2) from spoligo_post
The text was updated successfully, but these errors were encountered: