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The tools seems to have trouble to output the fastq file correctly if the bam file has low-coverage. In my case it is :
#rname startpos endpos numreads covbases coverage meandepth meanbaseq meanmapq chr1 1 249250621 11282 1049594 0.4211 0.00476702 26.9 63.4 chr2 1 243199373 12504 1133407 0.46604 0.00542827 26.9 62.9 chr3 1 198022430 9977 937568 0.473466 0.00534363 26.9 66.5 chr4 1 191154276 9223 857623 0.448655 0.00510673 26.9 62.4 chr5 1 180915260 8553 800982 0.442739 0.00499967 26.9 64.1 chr6 1 171115067 8724 816399 0.477105 0.00540157 26.9 66.1 chr7 1 159138663 7785 709773 0.446009 0.0051798 27 61.1 chr8 1 146364022 7599 705045 0.481706 0.00549357 27 64.8 chr9 1 141213431 6267 572471 0.405394 0.00468613 26.8 59.1 chr10 1 135534747 6815 636626 0.469714 0.00529091 26.9 64.2 chr11 1 135006516 6577 597143 0.442307 0.00513988 26.9 63.5 chr12 1 133851895 6635 612528 0.457616 0.00519541 26.9 65.3 chr13 1 115169878 4682 441733 0.383549 0.00429756 26.9 66.8 chr14 1 107349540 4414 412879 0.384612 0.00432871 26.9 63.9 chr15 1 102531392 3974 372428 0.363233 0.00407474 27 63.1 chr16 1 90354753 3946 359844 0.398257 0.00454397 27 61.4 chr17 1 81195210 3562 330014 0.406445 0.00457848 27 62.6 chr18 1 78077248 3998 357124 0.457398 0.00544089 26.9 64 chr19 1 59128983 2285 209688 0.354628 0.00399973 26.9 59.1 chr20 1 63025520 3107 289886 0.45995 0.00514412 26.9 67.1 chr21 1 48129895 1750 160523 0.33352 0.00379575 26.8 57.2 chr22 1 51304566 1511 136987 0.267007 0.00300574 26.7 56.7 chrX 1 155270560 4612 428987 0.276284 0.00307974 26.7 55.9 chrY 1 59373566 803 70312 0.118423 0.00139485 26.8 23.8 chrM 1 16569 213 3224 19.458 1.37884 27.2 65.9
which leads to the following output of bazam :
================================================================================ Bazam ================================================================================ bazam.Bazam [1] INFO |1:53:59 Extracting read pairs from some.bam bazam.Bazam [1] INFO |1:53:59 Initialising regions to scan from false gngs.pair.PairScanner [1] INFO |1:53:59 Beginning scan of some.bam gngs.pair.PairScanner [1] INFO |1:53:59 Created 4 read pair locators gngs.pair.PairScanner [1] INFO |1:54:00 Stopping parallel threads ... gngs.pair.PairScanner [1] INFO |1:54:00 Stopping Locator 0 gngs.pair.PairScanner [1] INFO |1:54:00 Stopping Locator 1 gngs.pair.PairScanner [1] INFO |1:54:00 Stopping Locator 2 gngs.pair.PairScanner [1] INFO |1:54:00 Stopping Locator 3 gngs.pair.PairScanner [1] INFO |1:54:00 Stopping Chimeric Locator gngs.pair.PairScanner [1] INFO |1:54:00 Stopping Formatter gngs.pair.PairScanner [1] INFO |1:54:00 Stopping Writer gngs.pair.PairScanner [1] INFO |1:54:00 Processed 142615 in 0.767 seconds @ 185455.14/s (*:0, loc: 142.6k,0,142.6k chimeric: 140.8k formatted: 0, written: 0)
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The tools seems to have trouble to output the fastq file correctly if the bam file has low-coverage. In my case it is :
which leads to the following output of bazam :
The text was updated successfully, but these errors were encountered: