diff --git a/openpype/hosts/nuke/plugins/publish/collect_slate_node.py b/openpype/hosts/nuke/plugins/publish/collect_slate_node.py
index 3baa0cd9b56..ac30bd6051a 100644
--- a/openpype/hosts/nuke/plugins/publish/collect_slate_node.py
+++ b/openpype/hosts/nuke/plugins/publish/collect_slate_node.py
@@ -17,7 +17,8 @@ def process(self, instance):
             (
                 n_ for n_ in nuke.allNodes()
                 if "slate" in n_.name().lower()
-                if not n_["disable"].getValue()
+                if not n_["disable"].getValue() and
+                "publish_instance" not in n_.knobs()  # Exclude instance nodes.
             ),
             None
         )
diff --git a/openpype/hosts/nuke/plugins/publish/collect_writes.py b/openpype/hosts/nuke/plugins/publish/collect_writes.py
index 6f9245f5b96..2bf43ed75a8 100644
--- a/openpype/hosts/nuke/plugins/publish/collect_writes.py
+++ b/openpype/hosts/nuke/plugins/publish/collect_writes.py
@@ -194,7 +194,6 @@ def _set_additional_instance_data(
                 "frameEndHandle": last_frame,
             })
 
-
         # TODO temporarily set stagingDir as persistent for backward
         # compatibility. This is mainly focused on `renders`folders which
         # were previously not cleaned up (and could be used in read notes)
@@ -269,10 +268,6 @@ def _get_existing_frames_representation(
             "tags": []
         }
 
-        frame_start_str = self._get_frame_start_str(first_frame, last_frame)
-
-        representation['frameStart'] = frame_start_str
-
         # set slate frame
         collected_frames = self._add_slate_frame_to_collected_frames(
             instance,