Skip to content

Commit

Permalink
added links to correspodning Rmd/md
Browse files Browse the repository at this point in the history
  • Loading branch information
friedue authored Sep 24, 2021
1 parent 9a55ec6 commit aad986b
Showing 1 changed file with 5 additions and 1 deletion.
6 changes: 5 additions & 1 deletion README.md
Original file line number Diff line number Diff line change
Expand Up @@ -69,6 +69,8 @@ The `TSCAN package (v.1.28.0)` was used to calculate pseudotime values and traje
GO term enrichments were calculated with the `clusterProfiler` package's functions `compareCluster()` and `enrichGO()` after excluding ribosomal genes from the gene lists of interest.
All plots were generated using `ggplot2` packages and the `pheatmap` package for heatmaps.

For details, see [processing_scRNAseq.md](scRNAseq/processing_scRNAseq.md), [processingVDJseq.md](scRNAseq/processingVDJseq.md), [figures_scRNAseq.Rmd](scRNAseq/figures_scRNAseq.Rmd) and [figures_VDJseq.Rmd](scRNAseeq/figures_VDJseq.Rmd).

## Integration with public scRNA-seq data sets

Gene count matrices for day 7 CD8 T cells from acute and chronic infection were obtained from GEO (GSE119940); using cell labels provided by Chen Yao we extracted the data for barcodes corresponding to memory precursor and memory-like cells as described in Yao et al. (2019).
Expand All @@ -78,6 +80,8 @@ For global comparisons of the different populations of T cells, we created pseud
These were then cpm-normalized via `edgeR::calcNormFactors` (Robinson 2010) and subsequently analyzed and visualized via PCA and hierarchical clustering using base R functions as well as `pcaExplorer::hi_loadings()` and the `dendextend` package (Marini 2019, Galili 2015).
All other analyses were done with the same principles and packages as described above.

For details see [Schauder2021.Rmd](scRNAseq/Schauder2021.Rmd), [Yao2019.Rmd](scRNAseq/Yao2019.Rmd), [figures_public_scRNAseq.Rmd](scRNAseq/figures_public_scRNAseq.Rmd) and the corresponding PDF/HTML files.

## References

* Amezquita, Robert, Lun, Aaron, Hicks, Stephanie, and Gottardo, Raphael (version 1.0.6). "Orchestrating Single-Cell Analysis with Bioconductor". <https://bioconductor.org/books/release/OSCA/>. Nat Methods. 2020 Feb;17(2):137-145. doi: 10.1038/s41592-019-0654-x. PMID: 31792435.
Expand Down Expand Up @@ -135,7 +139,7 @@ with Bioconductor." _F1000Res._, *5*, 2122. <https://doi.org/10.12688/f1000resea
The raw data (fastq files, read counts from CellRanger) can be downloaded from GEO (GSE151652).

For the single-cell data, some of the data can be downloaded from Box in the form of RDS (load into R via `in_data <- readRDS()`) or RDA objects (load into R via `load()`).
The `data/` directory in the scRNA-seq section contains some text files that contain just the cell labels and the mouse labels for individual cells.
The `data/` directory in the scRNA-seq directory contains some text files that contain just the cell labels and the mouse labels for individual cells.

| File_name | Robject_type | Details |
|--------------|-------------|------------|
Expand Down

0 comments on commit aad986b

Please sign in to comment.