Merge pull request #275 from iGEM-Engineering/update_did_not_builds

updated the builds for packages based on the synthesis tracker

CRISPR-Cas/README.md

@@ -14,36 +14,36 @@ Their associated guide RNA scaffold sequences as well as the position of the spa
     - CDS: 25
     - designed_sequence: 2
 - 1 vectors
-- 23 samples for distribution _<span style="color:red">2 parts not included</span>_
+- 12 samples for distribution _<span style="color:red">13 parts not included</span>_
 
 ### Parts:
 
 - D1005 (designed_sequence) in pSB1C5
 - D1006 (designed_sequence) in pSB1C5
-- K2558003 (CDS) in pSB1C5
+- K2558003 (CDS) _<span style="color:red">not included in distribution</span>_
 - ABE8_20_m (CDS) in pSB1C5
 - ABE8e_TadA_8e_V106W (CDS) in pSB1C5
-- ABEmax (CDS) in pSB1C5
+- ABEmax (CDS) _<span style="color:red">not included in distribution</span>_
 - AcrIIA2_bacterial (CDS) in pSB1C5
 - AcrIIA2_mammalian (CDS) in pSB1C5
 - AcrIIA4_bacterial (CDS) _<span style="color:red">not included in distribution</span>_
 - AcrIIA4_mammalian (CDS) _<span style="color:red">not included in distribution</span>_
-- BE4max (CDS) in pSB1C5
+- BE4max (CDS) _<span style="color:red">not included in distribution</span>_
 - CP1028_ABE8e (CDS) in pSB1C5
 - Cas9n_D10A (CDS) in pSB1C5
 - Cas9n_H840A (CDS) in pSB1C5
 - His_TwinStrep_SUMO_LwCas13a (CDS) in pSB1C5
-- LbCpf1_or_LbCas12a (CDS) in pSB1C5
-- LwCas13a (CDS) in pSB1C5
-- PE2 (CDS) in pSB1C5
-- SaABE8e_TadA_8e_V106W (CDS) in pSB1C5
-- SpCas9 (CDS) in pSB1C5
-- dLbCpf1_or_dLbCas12a (CDS) in pSB1C5
-- dSpCas9 (CDS) in pSB1C5
+- LbCpf1_or_LbCas12a (CDS) _<span style="color:red">not included in distribution</span>_
+- LwCas13a (CDS) _<span style="color:red">not included in distribution</span>_
+- PE2 (CDS) _<span style="color:red">not included in distribution</span>_
+- SaABE8e_TadA_8e_V106W (CDS) _<span style="color:red">not included in distribution</span>_
+- SpCas9 (CDS) _<span style="color:red">not included in distribution</span>_
+- dLbCpf1_or_dLbCas12a (CDS) _<span style="color:red">not included in distribution</span>_
+- dSpCas9 (CDS) _<span style="color:red">not included in distribution</span>_
 - dhLwCas13a (CDS) in pSB1C5
 - dhSpCas9 (CDS) in pSB1C5
 - hLwCas13a (CDS) in pSB1C5
-- hSaCas9 (CDS) in pSB1C5
+- hSaCas9 (CDS) _<span style="color:red">not included in distribution</span>_
 - hSpCas9 (CDS) in pSB1C5
 
 _Note: automatically generated from package Excel and sequence files; do not edit_

CRISPR-Cas/views/Libraries and Composites.csv

@@ -11,10 +11,10 @@ Interlab negative control,Transformation of a basic plasmid,,True,pSB1C3,,R0040,
 Libraries and Composite Parts,,Click on column headers for more information as to what to put into the column,,,,,,,,,,,,,,,,,,,,,,,
 "Short and human friendly name for the part, e.g., ""GFP Reporter""",Comments about design decisions. E.g. GC content or restriction sites removed,A description of how the part is meant to function and any information about the part that is not to do with how it was designed.,"True if this is a final product to be produced, false if this is an intermediate stage being specified for compactness","If the composite is being placed into a plasmid, provide the name of the plasmid. If the composite is being transformed into the genome, provide the name of the locus for incorporation.","Identity and strand constraints on parts, expressed as Part X RELATION Part Y, where RELATION is ""same as"", ""different from"", ""same orientation as"", ""different orientation from""","Part names (left to right: 5'->3') should match the names of other basic or composite parts. Add more columns as needed. Indicate alternatives with commas, RC(name) for reverse complement",,,,,,,,,,,,,,,,,,,
 Part/Library Name,Design Notes,Part Description,Final Product,Backbone/locus,Constraints,Part 1,Part 2,Part 3,Part 4,Part 5,Part 6,Part 7,Part 8,Part 9,,,,,,,,,,,
-Cas variants,,,True,pSB1C5,,D1005,"SpCas9, dSpCas9, hSpCas9, dhSpCas9, hLwCas13a, dhLwCas13a, LwCas13a, His_TwinStrep_SUMO_LwCas13a, LbCpf1 or LbCas12a, dLbCpf1 or dLbCas12a, dSpCas9_RFC10, ABE8.20-m, ABE8e TadA-8e V106W, ABEmax, BE4max, SaABE8e TadA-8e V106W, PE2, CP1028-ABE8e, hSaCas9",D1006,,,,,,,,,,,,,,,,,
+Cas variants,,,True,pSB1C5,,D1005,"hSpCas9, dhSpCas9, hLwCas13a, dhLwCas13a, His_TwinStrep_SUMO_LwCas13a, ABE8.20-m, ABE8e TadA-8e V106W, CP1028-ABE8e",D1006,,,,,,,,,,,,,,,,,
 Anti-CRISPR,,,True,pSB1C5,,D1005,"AcrIIA2_mammalian, AcrIIA2_bacterial",D1006,,,,,,,,,,,,,,,,,
 Nickases,,,True,pSB1C5,,D1005,"Cas9n_D10A, Cas9n_H840A",D1006,,,,,,,,,,,,,,,,,
-,,,False,,,,,,,,,,,,,,,,,,,,,,
-,,,False,,,,,,,,,,,,,,,,,,,,,,
+Did not build Cas variants,"Constructs belonged to original build plan, but did not synthesize due to Type IIS incompatible",,False,pSB1C5,,D1005,"dSpCas9_RFC10, LbCpf1 or LbCas12a, LwCas13a, SaABE8e TadA-8e V106W, SpCas9, dLbCpf1 or dLbCas12a, dSpCas9, hSaCas9",D1006,,,,,,,,,,,,,,,,,
+Unable to build Cas variants,"Constructs belonged to original build plan, but unable to synthesize as clonal product by TWIST",,False,pSB1C5,,D1005,"ABEmax, BE4max, PE2",D1006,,,,,,,,,,,,,,,,,
 ,,,False,,,,,,,,,,,,,,,,,,,,,,
 ,,,False,,,,,,,,,,,,,,,,,,,,,,